pcdna mruby2 addgene Search Results


pcdna3 1 clover mruby2  (ATCC)
90
ATCC pcdna3 1 clover mruby2
Pcdna3 1 Clover Mruby2, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mruby2  (Addgene inc)
93
Addgene inc mruby2
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 clover mruby2  (Addgene inc)
93
Addgene inc pcdna3 1 clover mruby2
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Pcdna3 1 Clover Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paper n a pcdna3 1 mruby2 rwnk1 1 494 tdp 43 lcd 273 414  (Addgene inc)
94
Addgene inc paper n a pcdna3 1 mruby2 rwnk1 1 494 tdp 43 lcd 273 414
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Paper N A Pcdna3 1 Mruby2 Rwnk1 1 494 Tdp 43 Lcd 273 414, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 clover  (Addgene inc)
92
Addgene inc pcdna3 clover
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Pcdna3 Clover, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna5  (Addgene inc)
93
Addgene inc pcdna5
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Pcdna5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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par sensor pcdna3 pbz mruby2  (Addgene inc)
93
Addgene inc par sensor pcdna3 pbz mruby2
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Par Sensor Pcdna3 Pbz Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mruby2 rfp vsfp cr  (Addgene inc)
93
Addgene inc mruby2 rfp vsfp cr
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Mruby2 Rfp Vsfp Cr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pcdna3  (Addgene inc)
94
Addgene inc plasmid pcdna3
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Plasmid Pcdna3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector prx2  (Addgene inc)
90
Addgene inc vector prx2
Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both <t>mRuby2-UCHD</t> and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )
Vector Prx2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both mRuby2-UCHD and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )

Journal: Nature Communications

Article Title: Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

doi: 10.1038/s41467-018-05851-9

Figure Lengend Snippet: Distinct dynamics of VE-cadherin, F-actin and ZO1 during JBL formation. a , b Still images (Supplementary Movie ) of an embryo showing the DLAV around 32 hpf in an embryo expressing both mRuby2-UCHD and VE-cad-Venus Tg ( fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;BAC ( cdh5:cdh5-Venus )). b A time series magnification of the inset in a . Individual channels are shown in inversed contrast. Similar observations were made in 11 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. c and d ) Still images of an embryo showing DLAV around 32 hpf (Supplementary Movie ) in an embryo expressing EGFP-ZO1 and mRuby2-UCHD ( Tg(fli:Gal4ff ubs3 ;UAS:mRuby2-UCHD ubs20 ;UAS:EGFP-hZO1 ubs5 ) ). Imaged at rate of 12 s/stack. Similar observations were made in 9 movies. Open arrow head points to established junctions and black arrowhead to pioneering junction. e Images of endothelial cells in a VE-cad-Venus expressing embryo injected with mCherry-ZO1 encoding plasmid Tg(BAC(cdh5:cdh5-ts)); fli1ep:mCherry-ZO1)) ( n = 7 embryos). f Close-up from panel e . Both channels are shown in inverted contrast. Scale bars 1 µm ( b – d ) and 10 µm ( a , e )

Article Snippet: The EGFP sequence of pT24xnrUAS:EGFP-UCHD was replaced by the sequence of mRuby2 (amplified from pcDNA3-mRuby2 was a gift from Michael Lin; Addgene plasmid #40260) or by the sequence of mClav2 (amplified from pmClavGR2-NT; Allele Biotechnology) to generate the final plasmids pT24xnrUAS:mRuby2-UCHD and pT24xnrUAS:mClav2-UCHD respectively.

Techniques: Expressing, Injection, Plasmid Preparation